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KMID : 0385219920020020103
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Korean Journal of Gerontology
1992 Volume.2 No. 2 p.103 ~ p.110
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Effects of O2- and H2O2 on the Function and Subtypes of Human Lymphocytes
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Son Myoung-Won
Lee Shee-Eun
Lee Byung-Soon
Jun Chang-Duk
Chung Hun-Taeg
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Abstract
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It is well known that reactive oxygen intermediates(ROIs) in phagocytic leukocytes have the microbicidal effects. Furthermore, serveral lines of evidence indicate that such ROIs as superoxide radical(O2-) and hydrogen peroxide(H2O2) are produced at the membrane surfaces of granulocytes and monocytes during phagocytosis and released into surrounding tissue. Released O2- and/or H2O2 may participate in damage to surrounding tissues or other blood cells. Immune attrition may occur in association with severe chronic infections and neoplastic disease. But the mechanisms of immunodefficiency in these disorders have not been elucidated precisely. In the present study, the effect of oxidant injury on membrane markers, functions and metabolism of lymphocytes were investigated. In order to approximate the type of oxidant stress that lymphocytes might encounter in vivo, these cells were exposed to the xanthine-xanthine oxidase system, and to differentiate the effects between O2- and H2O2, catalase was used to get rid of H2O2. Exposure of lymphocytes to ROIs slightly decreased such T cell marker positive cells as CD2, Cd3, CD4, and CD8-positive cells, and increased the CD19-positive B cells and HLA-DR, transferrin and CD25-synthesis of lymphocytes which had been stimulated by phytohemagglutinin(PHA). ROIs also inhibited the lymphocyte metabolism which was measured by deoxyglucose uptake after stimulation with PHA, but the effect of O2- was twofold compared to that of H2O2. The inhibitory effect of H2O2 on the cytotoxity of natural killer cells against K562 cells was stronger than that of O2- by two times. O2- was stronger than H2O2 in the inhibition of rosette formation between human lymphocytes and sheep red blood cells. These results strongly suggest that the immune dysfunction observed frequently by the ROIs which are released by the phagocytic cells of the host.
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KEYWORD
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ROI, xanthine-xanthine oxidase, catalase, PHA
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